This experiment was designed to study the problem whether diuretic activity appears in the affected rabbit sera. Korean domestic white male rabbits weighing 1.8 kg were used for this work. They were well hydrated orally by gavaging with 100 cc of 2 percent NaCL solution three times at one hour interval to maintain positive water balance through each study. Urine was collected through a indwelling cathether while the rabbits was placed on the holder. Sera was obtained once daily from -the rabbits of which ureter was ligated unilaterally or bilaterally.
When the urine volume through the indwelling catheter exceeded 3 ml per 5 min. after hydration, hydrated rabbits were given intravenously 1 ml of above serum and observed changes of urine volume per 5 min. for over 120 min.
The results were summerized as follows:
(1) No change of urine volume was noted in the hydrated rabbits after the injection of 1. 0 ml of serum of normal and hydrated rabbits.
(2) Sera obtained from the rabbits 24 hour, 2 and 3 day after ligation of both ureter, were given intravenously to hvdrated rabbits. Slight increase of urine volume was noted in the group of rabbits given 2 day serum.
(3) Sera obtained from the rabbits 24 hour, 2, 3, 4, 5, 6, 7, 8, 9,10, 12,15, 20, 25 and 30 day after ligation of one side ureter, were given intravenously to normal rabbits which were loaded with percent NaCI 3 times per Os An increase of urine volume was demonstrated in the groups of rabbits given 2 day to 10 except 9 day sera, but no remarkable change of urine volume was observed in the other groups of rabbits.
Judging from the results of these experiments, diuretic substances appeared in. the rabbit sera .induced by ligation of unilateral ureter.
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